Application

Anti-Caspase-4, clone 17D9 is an antibody against Caspase-4 for use in WB, IP, IH.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Caspase-4 in ischemia induced mouse brains (Kang, S., et al. (2000) JCB. 149(3):613-622).
Immunohistochemistry Analysis: A representative lot detected Caspase-4 in wildtype and ischemic mouse brains (Kang, S., et al. (2000) JCB. 149(3):613-622).

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Caspase-4 (CASP-4), also known as Caspase-11 (CASP-11), or Protease ICH-3, and encoded by the gene names Casp4, Casp11, Caspl, and Ich3, is a member of the peptidase C14A family and might be involved in ER-stress induced apoptosis. Caspase-4 protein has been shown to cleave Caspase-1 and might be involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-4 promotes IL-1 beta processing by ICE, and therefore may also play a role in inflammatory responses. Caspase-4 has been indicated to interact with TMEM214, in the process of ER membrane localization. Additionally, Caspase-4 is a heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The two subunits are derived by an autocatalytic mechanism, from the precursor sequence. Recent studies have shown that the activity of Caspase-4 is increased 30-fold by endotoxins (LPS). Other studies have indicated that Caspase-4 is induced by ER stress in a DDIT3/CHOP-dependent manner. Caspase-4 is expressed mostly in lung and spleen, and less in liver, skeletal muscle, kidney and testis.

Immunogen

Corresponding to mouse Caspase-4.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in bacterial J53 treated macrophage cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Caspase-4 in 10 µg of bacterial J53 treated macrophage cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~48 and 38 kDa observed